| # | Children | Name | Born | Died | Father | Mother |
|---|
| 1 | | | b. 18 Feb 1520 | d. 14 Apr 1521 | Manuel I King of Portugal | HABSBURG, Eleanor of Austria |
| 2 | | | b. 1521 | d. 1577 | Manuel I King of Portugal | HABSBURG, Eleanor of Austria |
| 3 | | | b. 6 Jun 1502 | d. 11 Jun 1557 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 4 | | | b. 4 Oct 1503 | d. 1 May 1539 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 5 | | | b. 31 Dec 1504 | d. 8 Jan 1538 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 6 | | | b. 3 Mar 1506 | d. 27 Nov 1555 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 7 | | | b. 5 Jun 1507 | d. 7 Nov 1534 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 8 | | | b. 23 Apr 1509 | d. 21 Apr 1540 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 9 | | | b. 31 Jan 1512 | d. 31 Jan 1580 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 10 | | | b. 1513 | d. 1513 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 11 | | | b. 7 Sep 1515 | d. 20 Sep 1540 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
| 12 | | | b. 9 Sep 1516 | d. 9 Sep 1516 | Manuel I King of Portugal | ARAGON, Maria of Queen of Portugal |
Puffer Genealogy
1469 - 1521 (52 years)